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Whole genome sequencing with the new technologies is a developing art that, despite the large volumes of data that can be produced, may still fail to provide a clear and thorough map of a genome. Plantagora: modeling whole genome sequencing and assembly of plant genomes.ĭirectory of Open Access Journals (Sweden)įull Text Available BACKGROUND: Genomics studies are being revolutionized by the next generation sequencing technologies, which have made whole genome sequencing much more accessible to the average researcher. A finished genome represents the genome assembly of much higher accuracy (with no gaps or incorrectly assembled areas) and quality (1 error/10,000 bp), validated through a number of computer and laboratory experiments.

The error rate of the consensus sequence produced at this stage is about 1/2000 bp. Despite the fact that automatically performed assembly (or draft assembly) is capable of covering up to 98% of the genome, in most cases, it still contains incorrectly assembled reads.

The genome assembly process can be divided into two steps: draft assembly and assembly improvement (finishing). In addition to the need to assemble millions of DNA fragments of different length (from 35 bp (Solexa) to 800 bp (Sanger)), great interest in analysis of microbial communities (metagenomes) of different complexities raises new problems and pushes some new requirements for sequence assembly tools to the forefront. Meanwhile the assembly of whole genome sequences remains a challenging task. Thanks to the variety of sequencing technologies available, the process of decoding the sequence of genomic DNA (or whole genome sequencing) has become robust and inexpensive. The analysis of the nucleotide sequence of DNA occupies a special place among the methods developed. Genome Sequence Databases (Overview): Sequencing and AssemblyĮnergy Technology Data Exchange (ETDEWEB)įrom the date its role in heredity was discovered, DNA has been generating interest among scientists from different fields of knowledge: physicists have studied the three dimensional structure of the DNA molecule, biologists tried to decode the secrets of life hidden within these long molecules, and technologists invent and improve methods of DNA analysis.

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